Efficient Artificial Fertilization and Ovulated Egg Preservation in Kawakawa Euthynnus affinis

Artificial fertilization of cultured fish is essential for seed production using breeding techniques. However, in tuna species, the success rate artificial tremendously low. In this study, it was reported that adequate procedure ovulated egg collection and storage kawakawa Euthynnus affinis. The eggs attempted new techniques disrupt only spawning activity without discontinuing ovulation. available time to use also examined by assessing optimal preservation process temperature. As a result, effectively executed thoroughly extracting immediately after ovulation, with 70% an ovulation 51.7%. Ovulated could be stored small quantities ovarian fluid sustain fertility. fertility better preserved Hanks’ solution. high productivity were achieved 3 h extraction when maintained solution at 20 °C, leading supply one-cell stage embryo microinjection treatment constantly continuously executing fertilization. This systematic permitted selective 1:1 mating between top-quality parental applying several developmental engineering breeding.